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Product Details
Synonyms
DEL1, EDIL3
Source
Human EDIL3, Fc Tag (ED3-H5259) is expressed from human 293 cells (HEK293). It contains AA Asp 24 - Glu 480 (Accession # O43854-1).
Predicted N-terminus: Asp 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 77.8 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human EDIL3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human EDIL3, Fc Tag (Cat. No. ED3-H5259) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT3-H82W9) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
Immobilized Biotinylated Human ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT3-H82W9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human EDIL3, Fc Tag (Cat. No. ED3-H5259) with a linear range of 0.3-39 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human EDIL3, Fc Tag (Cat. No. ED3-H5259) immobilized on CM5 Chip can bind Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52E3) with an affinity constant of 38.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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