This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 59.1 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Siglec-2 / CD22 (176-687), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Siglec-2 / CD22 (176-687), His Tag (Cat. No. SI2-H52H8) is more than 90% and the molecular weight of this protein is around 67-90 kDa verified by SEC-MALS.
Immobilized Human Siglec-2 / CD22 (176-687), His Tag (Cat. No. SI2-H52H8) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Siglec-2 Antibody, Human IgG1 with a linear range of 0.1-6 ng/mL (QC tested).
Monoclonal Anti-Human Siglec-2 Antibody, Human IgG1 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human Siglec-2 (176-687), His Tag (Cat. No. SI2-H52H8) with an affinity constant of 0.121 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Monoclonal Anti-Human CD22 Antibody, Human IgG1 on AHC Biosensor, can bind Human Siglec-2 / CD22 (176-687), His Tag (Cat. No. SI2-H52H8) with an affinity constant of 53 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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