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Your Position: Home > Immune Checkpoint Proteins > SIRP alpha > SIA-H5251

Human SIRP alpha / CD172a Protein, Fc Tag (MALS verified)

  • Synonym
    SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
  • Source
    Human SIRP alpha, Fc Tag (SIA-H5251) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Arg 370 (Accession # NP_001035111).
    Predicted N-terminus: Glu 31
  • Molecular Characterization
    Online(Glu 31 - Arg 370) NP_001035111

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 63.9 kDa. The protein migrates as 70-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) SDS-PAGE gel

Human SIRP alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human SIRP alpha, Fc Tag (Cat. No. ) MALS images

The purity of Human SIRP alpha, Fc Tag(Cat. No. SIA-H5251) was more than 90% and the molecular weight of this protein is around 140-155 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human SIRP alpha, Fc TagHuman SIRP alpha, Fc Tag (Cat. No. SIA-H5251) ELISA bioactivity

Immobilized Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) at 2 μg/mL (100 μL/well) can bind Human CD47, His Tag (Cat. No. CD7-H5227) with a linear range of 2-13 ng/mL (QC tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that Human SIRP alpha, Fc Tag (Cat. No. SIA-H5251) can bind to Jurkat cell expressing CD47. The concentration of SIRP alpha used is 1 μg/mL (Routinely tested).

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Order Details

Price(USD) : $260.00

Price(USD) : $1980.00

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Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

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