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Your Position: Home > Protein > IL-1 beta > ILB-H82E9

Biotinylated Human IL-1 beta / IL-1F2 Protein, His,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.

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  • Synonym
    IL1B,IL-1BETA,IL1F2,IL-1β
  • Source
    Biotinylated Human IL-1 beta, His,Avitag(ILB-H82E9) is expressed from human 293 cells (HEK293). It contains AA Ala 117 - Ser 269 (Accession # P01584-1 ).
    Predicted N-terminus: Ala 117
  • Molecular Characterization
    IL-1 beta Structure

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 21.0 kDa. The protein migrates as 26-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-1 beta SDS-PAGE

Biotinylated Human IL-1 beta, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-1 beta MALS images

The purity of Biotinylated Human IL-1 beta, His,Avitag (Cat. No. ILB-H82E9) is more than 90% and the molecular weight of this protein is around 20-28 kDa verified by SEC-MALS.

Bioactivity-ELISA
 IL-1 beta ELISA

Immobilized Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-1 beta, His,Avitag (Cat. No. ILB-H82E9) with a linear range of 0.3-10 ng/mL (QC tested).

  • Background
    Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta.
    IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects.
    IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
  • Clinical and Translational Updates

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