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Product Details
Synonyms
IL1B, IL-1BETA, IL1F2, IL-1β
Source
Biotinylated Human IL-1 beta, His,Avitag (ILB-H82E9) is expressed from human 293 cells (HEK293). It contains AA Ala 117 - Ser 269 (Accession # P01584-1).
Predicted N-terminus: Ala 117
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 21.0 kDa. The protein migrates as 26-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human IL-1 beta, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IL-1 beta, His,Avitag (Cat. No. ILB-H82E9) is more than 95% and the molecular weight of this protein is around 20-28 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-1 beta, His,Avitag (Cat. No. ILB-H82E9) with a linear range of 0.3-10 ng/mL (QC tested).
Protocol
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Background
IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects.
IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Recent Advances
Drug Development Progress- English Name：
Interleukin-1 beta
- Category：
- Approved Drugs：
10 Details
- Drugs in Clinical Trials：
20 Details
- Highest Development Stage：
Approved
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