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Product Details
Synonyms
TNFRSF9, 4-1BB, CD137, CDw137, ILA
Source
Biotinylated Human 4-1BB, Fc,Avitag (41B-H82F8) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Gln 186 (Accession # Q07011-1).
Predicted N-terminus: Leu 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 45.4 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human 4-1BB, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human 4-1BB, Fc,Avitag (Cat. No. 41B-H82F8) is more than 95% and the molecular weight of this protein is around 98-120 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human 4-1BB, Fc,Avitag (Cat. No. 41B-H82F8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind UreluMab, Human IgG4 with a linear range of 0.1-6 ng/mL (QC tested).
Protocol
Immobilized Human 4-1BB Ligand, Mouse IgG2a Fc Tag, low endotoxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human 4-1BB, Fc,Avitag (Cat. No. 41B-H82F8) with a linear range of 0.2-3 ng/mL (Routinely tested).
Protocol
Bioactivity-FACS
FACS assay shows that Biotinylated Human 4-1BB, Fc,Avitag (41B-H82F8) can bind to HEK293/Human 4-1BB Ligand / TNFSF9 Stable Cell Line (CHEK-ATP039). The concentration of Biotinylated Human 4-1BB, Fc,Avitag (41B-H82F8) used is 10 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 9
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
77 Details
- Highest Development Stage：
Phase 3 Clinical
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