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Your Position: Home > Agonist > 4-1BB Ligand > 41L-H5269

Human 4-1BB Ligand / TNFSF9 (71-254) Protein, Fc Tag, active trimer, premium grade

  • Synonym
    4-1BB Ligand,TNFSF9,CD137L
  • Source
    Human 4-1BB Ligand (71-254), premium grade(41L-H5269) is expressed from human 293 cells (HEK293). It contains AA Arg 71 - Glu 254 (Accession # P41273-1 ).
    Predicted N-terminus: Pro
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the N-terminus

    The protein has a calculated MW of 87.5 kDa. The protein migrates as 150-180 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Protein A
    <10 ppm of protein tested by ELISA.
  • Host Cell Protein
    <0.5 ng/μg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Mycoplasma
    Negative.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
4-1BB Ligand SDS-PAGE

Human 4-1BB Ligand (71-254), premium grade on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
4-1BB Ligand MALS images

The purity of Human 4-1BB Ligand (71-254), premium grade (Cat. No. 41L-H5269) is more than 90% and the molecular weight of this protein is around 182-222 kDa verified by SEC-MALS.

Bioactivity-ELISA
 4-1BB Ligand ELISA

Immobilized Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. 41B-H5256) at 2 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (71-254), premium grade (Cat. No. 41L-H5269) with a linear range of 0.1-6 ng/mL (QC tested).

 4-1BB Ligand ELISA

Immobilized Human 4-1BB, His Tag (Cat. No. 41B-H52Hc) at 2 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (71-254), premium grade (Cat. No. 41L-H5269) with a linear range of 0.1-3 ng/mL (Routinely tested).

 4-1BB Ligand ELISA

Immobilized Human 4-1BB Ligand (71-254), premium grade (Cat. No. 41L-H5269) at 2 μg/mL (100 μL/well) can bind Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. 41B-H5256) with a linear range of 0.2-3 ng/mL (Routinely tested).

Bioactivity-BLI
 4-1BB Ligand BLI

Loaded Human 4-1BB Ligand (71-254), premium grade (Cat. No. 41L-H5269) on Protein A Biosensor, can bind Human 4-1BB, His Tag (Cat. No. 41B-H52Hc) with an affinity constant of 425 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $400.00

Price(USD) : $3080.00

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