This protein carries a human IgG1 Fc tag at the N-terminus
The protein has a calculated MW of 87.5 kDa. The protein migrates as 150-180 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human 4-1BB Ligand (71-254), premium grade on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human 4-1BB Ligand (71-254), premium grade (Cat. No. 41L-H5269) is more than 90% and the molecular weight of this protein is around 182-222 kDa verified by SEC-MALS.
Immobilized Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. 41B-H5256) at 2 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (71-254), premium grade (Cat. No. 41L-H5269) with a linear range of 0.1-6 ng/mL (QC tested).
Immobilized Human 4-1BB, His Tag (Cat. No. 41B-H52Hc) at 2 μg/mL (100 μL/well) can bind Human 4-1BB Ligand (71-254), premium grade (Cat. No. 41L-H5269) with a linear range of 0.1-3 ng/mL (Routinely tested).
Immobilized Human 4-1BB Ligand (71-254), premium grade (Cat. No. 41L-H5269) at 2 μg/mL (100 μL/well) can bind Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. 41B-H5256) with a linear range of 0.2-3 ng/mL (Routinely tested).
Loaded Human 4-1BB Ligand (71-254), premium grade (Cat. No. 41L-H5269) on Protein A Biosensor, can bind Human 4-1BB, His Tag (Cat. No. 41B-H52Hc) with an affinity constant of 425 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $400.00
Price(USD) : $3080.00
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