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Your Position: Home > CAR-T > Mesothelin > MSN-HA2H6

APC-Labeled Human Mesothelin / MSLN (296-580) Protein, His TagStar Staining

  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    APC-Labeled Human Mesothelin (296-580), His Tag (MSN-HA2H6) is produced via conjugation of APC to Human Mesothelin (296-580), His Tag with a new generation site-specific technology under Star Staining labeling platform. Human Mesothelin (296-580), His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272.1 ).
    Predicted N-terminus: Glu 296
  • Molecular Characterization
    Mesothelin Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 46.5 kDa.

  • Conjugate

    APC

    Excitation Wavelength: 640 nm

    Emission Wavelength: 661 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-HTRF
 Mesothelin HTRF

Mix the 10 ng /well of Monoclonal Anti-Human MSLN Antibody, Human IgG1 with the Donor PAb Anti Human IgG-Eu cryptateHuman IgG1, incubated with the Acceptor APC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H6) at increasing concentration. Detection was performed with the EC50 of 0.6644 μg/mL (Routinely tested).

Evaluation of CAR expression
FACS Analysis of Anti-Mesothelin CAR Expression
 Mesothelin CAR_T

5e5 of anti-MSLN CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of APC-Labeled Human Mesothelin (296-580), His Tag (Cat. No.MSN-HA2H6) and negative control protein respectively (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). APC signal was used to evaluate the binding activity (QC tested).

FACS Analysis of Non-specific binding to PBMCs
 Mesothelin CAR_T

5e5 of PBMCs were stained with APC-Labeled Human Mesothelin (296-580), His Tag (Cat. No. MSN-HA2H6) and anti-CD3 antibody, washed and then analyzed with FACS. FITC signal was used to evaluate the expression of CD3+ T cells in PBMCs , and APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).

  • Background
    Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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