This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 138.5 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 Spike Trimer, His Tag (XD/BA.1 x AY.4 recombinant) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS-CoV-2 Spike Trimer, His Tag (XD/BA.1 x AY.4 recombinant) (Cat. No. SPN-C5226) is more than 95% and the molecular weight of this protein is around 551-608 kDa verified by SEC-MALS.
Immobilized SARS-CoV-2 Spike Trimer, His Tag (XD/BA.1 x AY.4 recombinant) (Cat. No. SPN-C5226) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-25 ng/mL (QC tested).
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer, His Tag (XD/BA.1 x AY.4 recombinant) (Cat. No. SPN-C5226) with a linear range of 0.1-13 ng/mL (Routinely tested).
Serial dilutions of Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (Cat. No. SPD-M265) were added into Human ACE2, Fc Tag (Cat. No. AC2-H525) : SARS-CoV-2 Spike Trimer, His Tag (XD/BA.1 x AY.4 recombinant) (Cat. No. SPN-C5226) binding reactions. The half maximal inhibitory concentration (IC50) is 0.1198 μg/mL (Routinely tested).
Serial dilutions of Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Omicron Specific) (Cat. No. SPD-M305) were added into Human ACE2, Fc Tag (Cat. No. AC2-H525) : SARS-CoV-2 Spike Trimer, His Tag (XD/BA.1 x AY.4 recombinant) (Cat. No. SPN-C5226) binding reactions. The half maximal inhibitory concentration (IC50) is 3.646 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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