Mouse APP / N-APP Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
APP-M52H3-100ug
$390.00
APP-M52H3-500ug
$1185.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    ABPP, APPI, Amyloid-beta A4 protein

  • Source

    Mouse N-APP, His Tag (APP-M52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 18 - Val 286 (Accession # P12023-1).

    Predicted N-terminus: Leu 18

    Request for sequence
  • Molecular Characterization

    APP Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 31.9 kDa. The protein migrates as 38-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    APP SDS-PAGE

    Mouse N-APP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    APP SEC-MALS

    The purity of Mouse N-APP, His Tag (Cat. No. APP-M52H3) is more than 95% and the molecular weight of this protein is around 30-45 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     APP ELISA

    Immobilized Mouse N-APP, His Tag (Cat. No. APP-M52H3) at 1 μg/mL (100 μL/well) can bind Aducanumab with a linear range of 2-78 ng/mL (QC tested).

    Protocol

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Background

Amyloid precursor protein (APP) is a type I integral membrane protein ubiquitously expressed in many tissues and concentrated in the synapses of neurons. The majority of APP is cleaved at the plasma membrane by the α-secretase in the non-amyloidogenic pathway. The amyloidogenic pathway starts with β-secretase cleavage by BACE1 on the N-terminal part of the Aβ domain, releasing sAPPβ from a membrane-anchored fragment named βCTF or C102, which is subsequently cleaved by γ-secretase to release Aβ.

Recent Advances

 
Drug Development Progress
  • English Name:

    Beta amyloid A4 protein

  • Category:

  • Approved Drugs:

    13 Details

  • Drugs in Clinical Trials:

    54 Details

  • Highest Development Stage:

    Approved

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