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Your Position: Home > Protein > R-Spondin 1 > RS1-H5269

Human R-Spondin 1 / RSPO1 (21-146) Protein, Fc Tag

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  • Synonym
    RSPO1,CRISTIN3,FLJ40906,RP11-566C13.1,R-spondin-1
  • Source
    Human R-Spondin 1 (21-146), Fc Tag(RS1-H5269) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ala 146 (Accession # Q2MKA7-1 ).
    Predicted N-terminus: Ser 21
  • Molecular Characterization
    R-Spondin 1 Structure

    This protein carries a human IgG1 Fc tag at the C-terminus

    The protein has a calculated MW of 40.2 kDa. The protein migrates as 46-56 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 52 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
R-Spondin 1 SDS-PAGE

Human R-Spondin 1 (21-146), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 R-Spondin 1 ELISA

Immobilized Human LRP-6 (20-630), Mouse IgG2a Fc Tag (Cat. No. LR6-H5253) at 5 μg/mL (100 μL/well) can bind Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) with a linear range of 0.008-0.5 μg/mL (QC tested).

 R-Spondin 1 ELISA

Immobilized Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) at 5 μg/mL (100 μL/well) can bind Human LGR5, His Tag (Cat. No. LG5-H52H3) with a linear range of 0.02-0.313 μg/mL (Routinely tested).

Bioactivity-Bioactivity CELL BASE
 R-Spondin 1 CELL

Human R-Spondin 1 (21-146), Fc Tag (Cat. No. RS1-H5269) induced TCF reporter activity in HEK293 cells. The EC50 for this effect is 0.0311 µg/mL (Routinely tested).

  • Background
    R-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), which is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus.
  • Clinical and Translational Updates

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