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Your Position: Home > Immune Checkpoint Proteins > Nectin-2 > CD2-H82E7

Biotinylated Human Nectin-2 / CD112 Protein, His,Avitag™ (MALS verified)

Recommended Hot Products: SP-11 Streptavidin Coated Plates, Clear, 96-Well  STN-N5116 Recombinant Streptavidin  SMB-B01 Magnetic Beads™ Streptavidin 
Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    CD112,Nectin-2,PVRL2
  • Source
    Biotinylated Human Nectin-2, His,Avitag (CD2-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).
    Predicted N-terminus: Gln 32
  • Molecular Characterization
    Online(Gln 32 - Leu 360) Q92692-2

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 39.1 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) SDS-PAGE gel

Biotinylated Human Nectin-2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human Nectin-2, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) is more than 95% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human Nectin-2, His,AvitagBiotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) ELISA bioactivity

Immobilized Biotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human PVRIG, Fc Tag (Cat. No. PVG-H5257) with a linear range of 0.6-10 ng/mL (QC tested).

Biotinylated Human Nectin-2, His,AvitagBiotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) ELISA bioactivity

Immobilized Biotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) with a linear range of 0.039-0.625 μg/mL (Routinely tested).

Biotinylated Human Nectin-2, His,AvitagBiotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) ELISA bioactivity

Immobilized Biotinylated Human Nectin-2, His,Avitag (Cat. No. CD2-H82E7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Nectin-3, Fc Tag (Cat. No. PV3-H5255) with a linear range of 0.039-0.625 μg/mL (Routinely tested).

  • Background
    Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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