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Product Details
Synonyms
ACE-2, ACEH, ACE2
Source
PE-Labeled Human ACE2, His Tag (AC2-HP2H3) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Ser 740 (Accession # Q9BYF1-1).
Predicted N-terminus: Gln 18
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA
Immobilized SARS S protein RBD, His Tag (Cat. No. SPD-S52H6) at 5 μg/mL (100 μL/well) can bind PE-Labeled Human ACE2, His Tag (Cat. No. AC2-HP2H3) with a linear range of 0.078-20 μg/mL (QC tested).
Protocol
Bioactivity-Flow Cytometry
Biotinylated SARS-CoV-2 Spike RBD Protein, His, Avitag™ (MALS verified) (Cat. No. SPD-C82E9) at 10pg/bead on Recombinant Streptavidin Protein (Cat. No. STN-N5116) at 2.5pg/bead were conjugated to fluorochrome-encoded microspheres. Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (AM359b) (MALS verified) (Cat. No. SPD-M265) competes with PE-Labeled Human ACE2, His tag (Cat. No. AC2-HP2H3) for binding to Biotinylated SARS-CoV-2 Spike RBD Protein encapsulated beads with a linear range of 16.25-8320 ng/mL.
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
Angiotensin-converting enzyme 2
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
7 Details
- Highest Development Stage：
Phase 2 Clinical
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