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Your Position: Home > Mutants > Nucleocapsid protein > NUN-C82E9

Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag™ (BA.2*&BA.3*/Omicron)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    Nucleocapsid protein,NP,Protein N
  • Source
    Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag™ (BA.2*&BA.3*/Omicron) (NUN-C82E9) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1(P13L, ERS31-33del, R203K, G204R, S413R)). The nucleocapsid mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2; GISAID clade: GRA; Nextstrain clade: 21L & Pango lineage: BA.3; GISAID clade: GRA; Nextstrain clade: 21M).
    Predicted N-terminus: Met 1
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 48.8 kDa. The protein migrates as 33 kDa and 50-60 kDa under reducing (R) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag™ (BA.2*&BA.3*/Omicron) (Cat. No. NUN-C82E9) SDS-PAGE gel

Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag™ (BA.2*&BA.3*/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag™ (BA.2*&BA.3*/Omicron)Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag™ (BA.2*&BA.3*/Omicron) (Cat. No. NUN-C82E9) ELISA bioactivity

Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (Cat. No. NUN-CH15) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag (BA.2*&BA.3*/Omicron) (Cat. No. NUN-C82E) with a linear range of 0.1-6 ng/mL (QC tested).

Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag™ (BA.2*&BA.3*/Omicron)Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag™ (BA.2*&BA.3*/Omicron) (Cat. No. NUN-C82E9) ELISA bioactivity

Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody, Human IgG1 (Cat. No. NUN-CH14) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Nucleocapsid protein, His,Avitag (BA.2*&BA.3*/Omicron) (Cat. No. NUN-C82E) with a linear range of 0.1-6 ng/mL (Routinely tested).

  • Background
    Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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