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Your Position: Home > CKP > NKG2A & CD94 > NC4-H82E4

Biotinylated Human NKG2A&CD94 Protein, His,Avitag™

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    NKG2A & CD94
  • Source
    Biotinylated Human NKG2A&CD94, His,Avitag (NC4-H82E4) is expressed from human 293 cells (HEK293). It contains AA Ala 113 - Leu 233 (NKG2A) & Asp 57 - Ile 179 (CD94) (Accession # P26715-1(NKG2A) & Q13241-1(CD94)).
    Predicted N-terminus: Ala 113
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 32.2 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human NKG2A&CD94, His,Avitag (Cat. No. NC4-H82E4) SDS-PAGE gel

Biotinylated Human NKG2A&CD94, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human NKG2A&CD94, His,AvitagBiotinylated Human NKG2A&CD94, His,Avitag (Cat. No. NC4-H82E4) ELISA bioactivity

Immobilized Anti-NKG2A Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human NKG2A&CD94, His,Avitag (Cat. No. NC4-H82E4) with a linear range of 0.1-4 ng/mL (QC tested).

  • Background
    CD94 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. NKG2A/CD159a is a transmembrane protein belonging to the CD94/NKG2 family of C-type lectin-like receptors that inhibits innate immune system activation. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA)-E on target cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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