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Product Details
Synonyms
IL2, TCGF, lymphokine, Interleukin 2
Source
Cynomolgus IL-2, His Tag (IL2-C5249) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 154 (Accession # Q29615-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 17.4 kDa. The protein migrates as 18 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Cynomolgus IL-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human IL-2 R alpha Protein, Fc Tag (Cat. No. ILA-H5251) at 5 μg/mL (100 μL/well) can bind Cynomolgus IL-2, His Tag (Cat. No. IL2-C5249) with a linear range of 20-78 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Cynomolgus IL-2 R beta, His Tag (Cat. No. ILB-C52H9) immobilized on CM5 Chip can bind Cynomolgus IL-2, His Tag (Cat. No. IL2-C5249) with an affinity constant of 377 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
K***s2023-05-18
LikesMy lab utilized this antibody as an analyte to prove/disprove binding capability to one of our proprietary antibodies. In addition to antibody screening, we also used it as a positive control to compare efficacy of in house IL2. We used it on Octet, which works with BLI technology to determine binding affinities. We appreciate the quick shipping and delivery of this product as our assays needed to be completed within a specific timeline.
Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-2
- Category：
- Approved Drugs：
2 Details
- Drugs in Clinical Trials：
46 Details
- Highest Development Stage：
Approved
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