Fluorescent Virus-Like Particle (VLP) Protein Isotype Control

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VLP-NF2P4-30ug
$125.00
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VLP-NF2P4-150ug
$410.00
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VLP-NF2P4
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  • Important Statement
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Application
  • Immunization
  • Cell based assay (FACS or functional assay)
  • Screening (ELISA)
Synonyms

VLP, Virus Like Particle, eVLP, VLPs

Source

Fluorescent Virus-Like Particle (VLP) Protein Isotype Control (VLP-NF2P4) is expressed from human 293 cells (HEK293).

Molecular Characterization

Fluorescent Virus-Like Particle (VLP) Protein Isotype Control (VLP-NF2P4) carries a GFP tag and is formed by self-assembly of envelop/capsid proteins from viruses, it can be used as isoytype control for Fluorescent VLPs displaying membrane proteins in various applications.

Structure

Virus-like particles (VLPs) are protein particles that mimic the shape of viruses but lack a viral genome, and incorporating transmembrane proteins on the external surface.

Synthesis Process

Envelope virus-like particles (eVLPs) are produced by co-expressing target membrane proteins with viral structural proteins in mammalian cells, enabling native embedding of membrane proteins into the lipid bilayer during VLP budding. This approach preserves authentic conformation, post-translational modifications, and orientation, while creating a completely detergent-free environment with repetitive multivalent display and strong immunogenicity making them ideal for immunization, binding assays in antibody discovery and screening (ELISA), and cell-based assays such as FACS, etc.

Conjugate

GFP

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 530 nm

Formulation

The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 3 months under sterile conditions.

Background

Virus-like particles (VLPs) are highly structured protein particles composed of single or multiple structural proteins of the virus. Its morphological structure is similar to that of natural virus particles, does not contain virus accounting, and has strong immunogenicity and biological activity. The empty shell structure of most VLP is icosahedral or helical structure, which is composed of several specific structural proteins. At present, it is widely used in vaccine production and antigen presentation.