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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 143.5 kDa. The protein migrates as 150-170 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CD109, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human CD109, His,Avitag (Cat. No. CD9-H82E4) is more than 85% and the molecular weight of this protein is around 150-180 kDa verified by SEC-MALS.
Biotinylated Human CD109, His,Avitag (Cat. No. CD9-H82E4) immobilized on CM5 Chip can bind Biotinylated Human TGF-Beta 1, Avitag (Cat. No. TG1-H8217) with an affinity constant of 0.248 μM as determined in a SPR assay (Biacore 8K) (QC tested).
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