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Product Details
Synonyms
Spike, S protein, Spike glycoprotein, S glycoprotein
Source
SARS-CoV-2 Spike Trimer, His Tag (BA.2/Omicron) (SPN-C522b) is expressed from human 293 cells (HEK293). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2; GISAID clade: GRA; Nextstrain clade: 21L). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 138.0 kDa. The protein migrates as 160-190 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Supplied as 0.2 μm filtered solution in 0.1 M Sodium citrate, pH5.5.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
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Performance Data
SDS-PAGE
SARS-CoV-2 Spike Trimer, His Tag (BA.2/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of SARS-CoV-2 Spike Trimer, His Tag (BA.2/Omicron) (Cat. No. SPN-C522b) is more than 90% and the molecular weight of this protein is around 490-540 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.2/Omicron) (Cat. No. SPN-C522b) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.2/Omicron) (Cat. No. SPN-C522b) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.2-6 ng/mL (Routinely tested).
Protocol
Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.2/Omicron) (Cat. No. SPN-C522b) at 5 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Omicron Specific) (Cat. No. SPD-M305) with a linear range of 0.2-6 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Coronavirus spike glycoprotein
- Category：
- Approved Drugs：
15 Details
- Drugs in Clinical Trials：
88 Details
- Highest Development Stage：
Approved
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