Human IL-39 Protein, Fc Tag (MALS verified)

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Cat. No. / Size
Price
Qty
IL9-H5259-100ug
$360.00
IL9-H5259-1mg (200ug X 5)
$2380.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    EBI3, IL39, IL-39EBI3, IL39p19, Interleukin-39, IL-39

  • Source

    Human IL-39, Fc Tag (IL9-H5259) is expressed from human 293 cells (HEK293). It contains AA Arg 21 - Lys 229 & Arg 20 - Pro 189 (Accession # Q14213-1 (IL-27 beta) & Q9NPF7-1 (IL-23 alpha)).

    Predicted N-terminus: Arg 21

  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 69.4 kDa. The protein migrates as 65-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 360 mM NaCl, PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-39 SDS-PAGE

    Human IL-39, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    IL-39 SEC-MALS

    The purity of Human IL-39, Fc Tag (Cat. No. IL9-H5259) is more than 85% and the molecular weight of this protein is around 140-170 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     IL-39 ELISA

    Immobilized Human IL-39, Fc Tag (Cat. No. IL9-H5259) at 1 μg/mL (100 μL/well) can bind Anti-IL23A MAb with a linear range of 0.02-0.5 μg/mL (QC tested).

    Protocol

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Background

Interleukin 39 (IL-39) is a new member of the IL-12 family and is composed of IL-23p19 and Ebi3 subunits.The two subunits, IL-23p19 and Ebi3, are shared with IL-23 and IL-27/IL-35. IL-23p19 is a four-helix bun dle and has homology with IL-6 and granulocyte colony stimulating factor. Ebi3 was first identified in B lympho cytes infected with EB virus. Some studies have reported that The combination of IL-23R and gp130 forms the IL-39 receptor. At present, a research on IL-39 is still in its initial stage, and many issues have not been solved.

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