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Your Position: Home > Cytokines > IL-1RL1 > IL1-H82E9

Biotinylated Human IL-1RL1 / ST2 Protein, His,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    IL1RL1,DER4,FIT-1,IL33R,ST2,ST2L,ST2V,T1
  • Source
    Biotinylated Human IL-1RL1, His,Avitag™ (IL1-H82E9) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - Pro 323 (Accession # Q01638-1).
    Predicted N-terminus: Lys 19
  • Molecular Characterization
    Online(Lys 19 - Pro 323) Q01638-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 38.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-1RL1, His,Avitag™ (Cat. No. IL1-H82E9) SDS-PAGE gel

Biotinylated Human IL-1RL1, His,Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human IL-1RL1, His,Avitag™ (Cat. No. ) MALS images

The purity of Biotinylated Human IL-1RL1, His,Avitag™ (Cat. No. IL1-H82E9) is more than 90% and the molecular weight of this protein is around 58-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human IL-1RL1, His,Avitag™Biotinylated Human IL-1RL1, His,Avitag™ (Cat. No. IL1-H82E9) ELISA bioactivity

Immobilized Human IL-33, His Tag (Cat. No. IL3-H52H7) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-1RL1, His,Avitag (Cat. No. IL1-H82E9) with a linear range of 0.3-10 ng/mL (QC tested).

  • Background
    Interleukin 1 receptor-like 1 (IL1RL1) is also known as ST2, DER4, FIT-1, IL33R, ST2L, ST2V, which belongs to the interleukin-1 receptor family. IL1RL1 contains three Ig-like C2-type (immunoglobulin-like) domains and one TIR domain. IL1RL1 is receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL1RL1 possibly involved in helper T-cell function. IL1RL1 can interact with MYD88, IRAK1, IRAK4, and TRAF6.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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