Biotinylated Human AXL Protein, Fc,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
AXL-H82F9-25ug
$360.00
AXL-H82F9-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    AXL, UFO

  • Source

    Biotinylated Human AXL, Fc,Avitag (AXL-H82F9) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Trp 451 (Accession # P30530-1).

    Predicted N-terminus: Ala 26

    Request for sequence
  • Molecular Characterization

    Axl Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 74.3 kDa. The protein migrates as 90-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Axl SDS-PAGE

    Biotinylated Human AXL, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Axl SEC-MALS

    The purity of Biotinylated Human AXL, Fc,Avitag (Cat. No. AXL-H82F9) is more than 95% and the molecular weight of this protein is around 155-185 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     Axl ELISA

    Immobilized Human GAS6, His Tag (Cat. No. GA6-H5249) at 5 μg/mL (100 μL/well) can bind Biotinylated Human AXL, Fc,Avitag (Cat. No. AXL-H82F9) with a linear range of 1-31 ng/mL (QC tested).

    Protocol

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Background

AXL Receptor Tyrosine Kinase is also known as Tyrosine-protein kinase receptor UFO, which belongs to the protein kinase superfamily, Tyr protein kinase family and AXL/UFO subfamily. AXL contains two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. AXL is highly expressed in metastatic colon tumors. AXL is activated by GAS6-binding and subsequent autophosphorylation. AXL is involved in signal transduction from the extracellular matrix into the cytoplasm by binding growth factors, and thus implicated in the stimulation of cell proliferation.

Recent Advances

 
Drug Development Progress
  • English Name:

    AXL receptor tyrosine kinase

  • Category:

  • Approved Drugs:

    2 Details

  • Drugs in Clinical Trials:

    29 Details

  • Highest Development Stage:

    Approved

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