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Your Position: Home > CD&BIOMARKER > CD117 > CD7-C52H9

Cynomolgus CD117 / c-kit Protein, His Tag (MALS verified)

  • Synonym
    CD117,SCFR,c-Kit,KIT
  • Source
    Cynomolgus CD117, His Tag (CD7-C52H9) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Thr 521 (Accession # XP_005555329.1).
    Predicted N-terminus: Gln 26
  • Molecular Characterization
    Online(Gln 26 - Thr 521) XP_005555329.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 57.6 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus CD117, His Tag (Cat. No. CD7-C52H9) SDS-PAGE gel

Cynomolgus CD117, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Cynomolgus CD117, His Tag (Cat. No. ) MALS images

The purity of Cynomolgus CD117, His Tag (Cat. No. CD7-C52H9) is more than 90% and the molecular weight of this protein is around 68-88 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus CD117, His TagCynomolgus CD117, His Tag (Cat. No. CD7-C52H9) ELISA bioactivity

Immobilized Cynomolgus CD117, His Tag (Cat. No. CD7-C52H9) at 2 μg/mL (100 μL/well) can bind Biotinylated Human SCF, Avitag,His Tag (Cat. No. SCF-H82E1) with a linear range of 0.2-5 ng/mL (QC tested).

  • Background
    Mast/stem cell growth factor receptor Kit (c-Kit), a member of the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily, is also known as piebald trait protein (PBT), p145 c-kit, tyrosine-protein kinase Kit and CD117,which contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, CD117 can activate several signaling pathways. Furthermore, Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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