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Product Details
Synonyms
LAG3, CD223, FDC
Source
Biotinylated Human LAG-3, His,Avitag (LA3-H82E9) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1).
Predicted N-terminus: Leu 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 49.8 kDa. The protein migrates as 60-63 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human LAG-3, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human LAG-3, His,Avitag (Cat. No. LA3-H82E9) is more than 95% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human LAG-3, His,Avitag (Cat. No. LA3-H82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human LAG3 Antibody, Human IgG1 with a linear range of 0.5-2 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews
N***r2022-11-08
LikesOrdered this reagent for a binding ELISA performed for a client. Client had questions about extending the reagent expiry and getting extra documentation provided for a GMP project. Acros customer service was great in answering all of my questions and providing documentation within just a few mins. I was provided with the COA and a memo ensuring the expiry could be extended from data that Acros had collected. I was also able to talk to other scientists on the technical side of their customer services and ensure that what I was relaying to the client was accurate. Really helped out on this project and the client was very happy! Thank you Acros!
Background
Recent Advances
Drug Development Progress- English Name：
Lymphocyte activation gene 3 protein
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
32 Details
- Highest Development Stage：
Approved
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