Biotinylated SARS-CoV-2 Spike S2 protein, His,Avitag™ (BA.1/Omicron)

Synonyms

Spike, S2 protein, Spike glycoprotein Subunit2, S glycoprotein Subunit2, Spike protein S2

Source

Biotinylated SARS-CoV-2 Spike S2 protein, His,Avitag™ (BA.1/Omicron) (S2N-C82E3) is expressed from human 293 cells (HEK293). It contains AA Ser Ser 686 - Pro 1213 (Accession # QHD43416.1 (N764K, D796Y, N856K, Q954H, N969K, L981F, F817P, A892P, A899P, A942P, K986P, V987P)). The mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.1; GISAID clade: GRA; Nextstrain clade: 21K). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to prevent the formation of aggregates in the course of protein production.

Predicted N-terminus: Ser 686

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 61.6 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process