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Your Position: Home > Mutants > Spike S2 > S2N-C82E4

Biotinylated SARS-CoV-2 Spike S2 protein, His,Avitag™ (BA.2/Omicron)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    Spike,S2 protein,Spike glycoprotein Subunit2,S glycoprotein Subunit2,Spike protein S2
  • Source
    Biotinylated SARS-CoV-2 Spike S2 protein, His,Avitag™ (BA.2/Omicron) (S2N-C82E4) is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1 (N764K, D796Y, Q954H, N969K, F817P, A892P, A899P, A942P, K986P, V987P)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.2/3/4/5). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to prevent the formation of aggregates in the course of protein production.
    Predicted N-terminus: Ser 686
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)

    The protein has a calculated MW of 61.7 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike S2 SDS-PAGE

Biotinylated SARS-CoV-2 Spike S2 protein, His,Avitag (BA.2/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Spike S2 ELISA

Immobilized Biotinylated SARS-CoV-2 Spike S2 protein, His,Avitag (BA.2/Omicron) (Cat. No. S2N-C82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike S2 protein Antibody, Human IgG4 (Cat. No. S2N-S86) with a linear range of 0.1-1 ng/mL (QC tested).

  • Background
    Its been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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