Product Details
Synonyms
OX40L, TNFSF4, CD252, Glycoprotein Gp34, TXGP1, CD134 ligand, CD134L
Source
Rabbit OX40 Ligand, His Tag (OXL-R5249) is expressed from human 293 cells (HEK293). It contains AA Gln 45 - Pro 187 (Accession # O02765-1).
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 51.2 kDa. The protein migrates as 50-60 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Rabbit OX40 Ligand, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Rabbit OX40 Ligand, His Tag (Cat. No. OXL-R5249) is more than 95% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Rabbit OX40 Ligand, His Tag (Cat. No. OXL-R5249) at 2 μg/mL (100 μL/well) can bind Human OX40, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. OX0-H5252) with a linear range of 1-39 ng/mL (QC tested).
Protocol
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Background
Recent Advances
- English Name:
Tumor necrosis factor ligand superfamily member 4
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
13 Details
- Highest Development Stage:
Phase 3 Clinical
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