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Your Position: Home > Enzyme > DPPIV > DP4-C52H9

Cynomolgus DPPIV / CD26 Protein, His Tag, active dimer (active enzyme, MALS verified)

  • Synonym
    DPP4,ADABP,ADCP2,CD26,DPPIV,TP103
  • Source
    Cynomolgus DPPIV, His Tag (DP4-C52H9) is expressed from human 293 cells (HEK293). It contains AA Asn 29 - Pro 766 (Accession # F6VRB0-1).
    Predicted N-terminus: Asn 29
  • Molecular Characterization
    Online(Asn 29 - Pro 766) F6VRB0-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 86.9 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 150 mM NaCl, pH8.0 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus DPPIV, His Tag (Cat. No. DP4-C52H9) SDS-PAGE gel

Cynomolgus DPPIV, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Cynomolgus DPPIV, His Tag (Cat. No. ) MALS images

The purity of Cynomolgus DPPIV, His Tag (Cat. No. DP4-C52H9) is more than 90% and the molecular weight of this protein is around 185-215 kDa verified by SEC-MALS.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >3500 pmol/min/μg(QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

MERS S1 protein, His Tag (Cat. No. S1N-M52H5) immobilized on CM5 Chip can bind Cynomolgus DPPIV, His Tag (Cat. No. DP4-C52H9) with an affinity constant of 60.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Dipeptidyl peptidase-IV (DPPIV) is also known as adenosine deaminase complexing protein 2, DPPIV or CD26 is antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. The substrates of DPPIV are proline (or alanine)-containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides. DPPIV plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. DPPIV plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others. DPPIV also binds the enzyme adenosine deaminase specifically and with high affinity. The significance of this interaction has yet to be established.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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