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Your Position: Home > TME Relevant Pathway > DLL3 > DL3-M52H9

Mouse DLL3 Protein, His Tag

  • Synonym
    Delta3,delta-like 3 (Drosophila),delta-like protein 3,DLL3,Pudgy,SCDO1,SCDO1delta3
  • Source
    Mouse DLL3, His Tag (DL3-M52H9) is expressed from human 293 cells (HEK293). It contains AA Ala 25 - Arg 488 (Accession # O88516-1).
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus and a fusion design at the N-terminus. The protein has a calculated MW of 118 kDa. The protein migrates as 110-120 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse DLL3, His Tag (Cat. No. DL3-M52H9) SDS-PAGE gel

Mouse DLL3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Mouse DLL3, His TagMouse DLL3, His Tag (Cat. No. DL3-M52H9) ELISA bioactivity

Immobilized Mouse DLL3, His Tag (Cat. No. DL3-M52H9) at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding DSL of DLL3), Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Mouse DLL3, His TagMouse DLL3, His Tag (Cat. No. DL3-M52H9) ELISA bioactivity

Immobilized Mouse DLL3, His Tag (Cat. No. DL3-M52H9) at 1 μg/mL (100 μL/well) can bind Anti-DLL3 Antibody (specific Binding EGF5 of DLL3), Human IgG1 with a linear range of 0.1-2 ng/mL (Routinely tested).

  • Background
    Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands.May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. DLL3 protein is expressed on the surface of tumor cells but not in normal adult tissues.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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