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Your Position: Home > Aneuro > Glypican 4 > GP4-H52H3

Human Glypican 4 / GPC4 Protein, His Tag (MALS verified)

  • Synonym
    Glypican 4,glypican proteoglycan 4,glypican-4,GPC4,K-glypican
  • Source
    Human Glypican 4, His Tag(GP4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - Ser 529 (Accession # O75487-1 ).
  • Molecular Characterization
    Glypican 4 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 59.5 kDa.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glypican 4 SDS-PAGE

Human Glypican 4, His Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Glypican 4 MALS images

The purity of Human Glypican 4, His Tag(Cat. No. GP4-H52H3) is more than 90% and the molecular weight of this protein is around 55-69 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Glypican 4 ELISA

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 1 μg/mL (100 μL/well) can bind Human Glypican 4, His Tag (Cat. No. GP4-H52H3) with a linear range of 0.02-0.625 μg/mL (QC tested).

  • Background
    Glypican-4 is a protein that in humans is encoded by the GPC4 gene. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3 end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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