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Your Position: Home > Signal Transduction > STAT3 > ST3-H5149

Human STAT3 Protein, His Tag (MALS & SPR verified)

  • Synonym
    STAT3, signal transducer and activator of transcription 3
  • Source
    Human STAT3, His Tag(ST3-H5149) is expressed from E.coli cells. It contains AA Gly 127 - Arg 688 (Accession # P40763-1 ).
    Predicted N-terminus: Met
  • Molecular Characterization
    STAT3 Structure

    This protein carries a polyhistidine tag at the N-terminus

    The protein has a calculated MW of 66.4 kDa. The protein migrates as 66-70 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
STAT3 SDS-PAGE

Human STAT3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
STAT3 MALS images

The purity of Human STAT3, His Tag (Cat. No. ST3-H5149) is more than 85% and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.

Bioactivity-SPR
 STAT3 SPR

Human STAT3, His Tag (Cat. No. ST3-H5149) immobilized on CM5 Chip can bind STAT3 degrader with an affinity constant of 44.6 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Bioactivity-BLI
 STAT3 BLI

Loaded Human STAT3, His Tag (Cat. No. ST3-H5149) on NTA Biosensor, can bind STAT3 degrader with an affinity constant of 29.4 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Signal transducer and activator of transcription 3 (STAT3) regulates the expression of genes important for essential cellular processes including survival, proliferation, self-renewal, angiogenesis, and immune response. As an early tumor diagnostic marker, it is considered that constitutive STAT3 activation is a sufficient factor to cause malignant transformation and tumor development in model systems. The classical IL-6/STAT3 signaling pathway in cancer cells. IL-6 binds to the membrane-bound IL-6 receptor α (IL-6R) and IL-6 receptor β (also known as gp130). The IL-6/IL-6R/gp130 complex activate the phosphorylation of JAKs, followed by STAT3 phosphorylation and activation. Then, phosphorylated STAT3 forms a homodimer and translocates into the nucleus to bind to the promotor region of target genes and activates target gene transcription.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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