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Your Position: Home > FcR > FcRn (FCGRT & B2M) > FCM-C52W8

Canine FcRn / FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (MALS & SPR verified)

  • Synonym
    FcRn,FCGRT & B2M
  • Source
    Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free(FCM-C52W8) is expressed from human 293 cells (HEK293). It contains AA Ala 23 - Ser 298 (FCGRT) & Val 28 - Asn 125 (B2M) (Accession # XP_038512242.1 (FCGRT) & NP_001271408.1 (B2M)).
    Predicted N-terminus: Ala 23
  • Molecular Characterization
    FcRn (FCGRT & B2M) Structure

    The protein has a calculated MW of 32.3 kDa (FCGRT) & 11.6 kDa (B2M). The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with Trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FcRn (FCGRT & B2M) SDS-PAGE

Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
FcRn (FCGRT & B2M) MALS images

The purity of Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free(Cat. No. FCM-C52W8) is more than 90% and the molecular weight of this protein is around 43-60 kDa verified by SEC-MALS.

Bioactivity-SPR
 FcRn (FCGRT & B2M) SPR

Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) captured on CM5 chip via anti-His antibody can bind Herceptin with an affinity constant of 0.171 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

 FcRn (FCGRT & B2M) SPR

Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) captured on CM5 chip via anti-His antibody can bind Ipilimumab with an affinity constant of 0.132 μM as determined in a SPR assay (Biacore 8K) (QC tested).

 FcRn (FCGRT & B2M) SPR

Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with an affinity constant of 1.11 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51 (equivalent to FCGRT), and p14 (equivalent to beta-2-microglobulin), and forms an MHC class I-like heterodimer. Fc fragment of IgG, receptor, transporter, alpha (FCGRT) binds to the Fc region of monomeric immunoglobulins gamma and mediates the uptake of IgG from milk. FCGRT possible role in transfer of immunoglobulin G from mother to fetus. Beta-2-microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC) and involved in the presentation of peptide antigens to the immune system.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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