Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.3 kDa (FCGRT) and 11.6 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 35-37 kDa (FCGRT) and 13 kDa (B2M) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) is more than 90% and the molecular weight of this protein is around 43-60 kDa verified by SEC-MALS.
Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) captured on CM5 chip via anti-His antibody can bind Herceptin with an affinity constant of 0.171 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) captured on CM5 chip via anti-His antibody can bind Ipilimumab with an affinity constant of 0.132 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Canine FCGRT&B2M Heterodimer Protein, His Tag&Tag Free (Cat. No. FCM-C52W8) captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3) with an affinity constant of 1.11 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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