This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.2 kDa. The protein migrates as 44-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Canine B7-H4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Canine B7-H4, His Tag (Cat. No. B74-C52H8) is more than 95% and the molecular weight of this protein is around 38-50 kDa verified by SEC-MALS.
Immobilized Monoclonal Anti-Human B7-H4 Antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Canine B7-H4, His Tag (Cat. No. B74-C52H8) with a linear range of 1-16 ng/mL (QC tested).
Anti-B7-H4 antibody, Human IgG1 captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Canine B7-H4, His Tag (Cat. No. B74-C52H8) with an affinity constant of 8.59 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $350.00
Price(USD) : $2310.00
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