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Your Position: Home > Agonist > B7-1 > B71-R52H9

Rat B7-1 / CD80 Protein, His Tag

  • Synonym
    CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7
  • Source
    Rat B7-1, His Tag(B71-R52H9) is expressed from human 293 cells (HEK293). It contains AA Ile 39 - Gln 248 (Accession # Q62680-1 ).
    Predicted N-terminus: Ile 39
  • Molecular Characterization
    B7-1 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 25.8 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with Trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
B7-1 SDS-PAGE

Rat B7-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
 B7-1 ELISA

Immobilized Mouse ICOS (C137S, C138S), His Tag (Cat. No. ICS-M52H8) at 5 μg/mL (100 μL/well) can bind Rat B7-H2, Fc Tag (Cat. No. B72-R5259) with a linear range of 0.6-39 ng/mL (QC tested).

 B7-1 ELISA

Immobilized Biotinylated Human ICOS (C136S, C137S), His,Avitag (recommended for biopanning) (Cat. No. ICS-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Rat B7-H2, Fc Tag (Cat. No. B72-R5259) with a linear range of 2-39 ng/mL (Routinely tested).

  • Background
    B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response.
    B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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