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Your Position: Home > Cytokines > CCL1 > CC1-H82E9

Biotinylated Human CCL1 / I-309 / C-C motif chemokine 1 Protein, His,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    CCL1/I-309/C-C motif chemokine 1
  • Source
    Biotinylated Human CCL1, His,Avitag (CC1-H82E9) is expressed from human 293 cells (HEK293). It contains AA Lys 24 - Lys 96 (Accession # P22362-1).
    Predicted N-terminus: Lys 24
  • Molecular Characterization
    Online(Lys 24 - Lys 96) P22362-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 12.1 kDa. The protein migrates as 15 kDa and 17-19 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CCL1, His,Avitag (Cat. No. CC1-H82E9) SDS-PAGE gel

Biotinylated Human CCL1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human CCL1, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human CCL1, His,Avitag (Cat. No. CC1-H82E9) is more than 90% and the molecular weight of this protein is around 18-26 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human CCL1, His,AvitagBiotinylated Human CCL1, His,Avitag (Cat. No. CC1-H82E9) ELISA bioactivity

Immobilized Biotinylated Human CCL1, His,Avitag (Cat. No. CC1-H82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CCR8, Flag,His Tag with a linear range of 0.3-39 ng/mL (QC tested).

  • Background
    Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL1, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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