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Product Details
Synonyms
Integrin alpha 1 beta 1, ITGA1&ITGB1
Source
Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-R52W7) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Pro 1142 (ITGA1) & Gln 21 - Asp 729 (ITGB1) (Accession # P18614-1 (ITGA1) & P49134-1 (ITGB1)).
Predicted N-terminus: Phe 29 (ITGA1) & Gln 21 (ITGB1)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA1 and ITGB1, has a calculated MW of 130.0 kDa (ITGA1) and 83.7 kDa (ITGB1). Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-R52W7) with a linear range of 0.02-0.313 μg/mL (QC tested).
Protocol
Immobilized Human Collagen IV Protein at 2 μg/mL (100 μL/well) can bind Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-R52W7) with a linear range of 0.002-0.156 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Integrin alpha1/beta1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
0 Details
- Highest Development Stage：
Pending
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