Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA1 and ITGB1, has a calculated MW of 130.0 kDa (ITGA1) and 83.7 kDa (ITGB1). Subunit ITGA1 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-R52W7) with a linear range of 0.02-0.313 μg/mL (QC tested).
Immobilized Human Collagen IV Protein at 2 μg/mL (100 μL/well) can bind Rat ITGA1&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-R52W7) with a linear range of 0.002-0.156 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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