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This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 36.4 kDa. The protein migrates as 52-62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human MICA, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human MICA, His,Avitag (Cat. No. MIA-H82E6) is more than 90% and the molecular weight of this protein is around 47-64 kDa verified by SEC-MALS.
Immobilized Biotinylated Human MICA, His,Avitag (Cat. No. MIA-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human NKG2D, Fc Tag (Cat. No. NKD-H5265) with a linear range of 0.3-10 ng/mL (QC tested).
Immobilized Human NKG2D, Fc Tag (Cat. No. NKD-H5265) at 2 μg/mL (100 μL/well) can bind Biotinylated Human MICA, His,Avitag (Cat. No. MIA-H82E6) with a linear range of 0.3-39 ng/mL (Routinely tested).
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