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Your Position: Home > Others > MICA > MIA-H82E6

Biotinylated Human MICA Protein, His,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    MIC-A
  • Source
    Biotinylated Human MICA, His,Avitag (MIA-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 24 - Gln 308 (Accession # Q29983-1).
    Predicted N-terminus: Glu 24
  • Molecular Characterization
    Online(Glu 24 - Gln 308) Q29983-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 36.4 kDa. The protein migrates as 52-62 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human MICA, His,Avitag (Cat. No. MIA-H82E6) SDS-PAGE gel

Biotinylated Human MICA, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human MICA, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human MICA, His,Avitag (Cat. No. MIA-H82E6) is more than 90% and the molecular weight of this protein is around 47-64 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human MICA, His,AvitagBiotinylated Human MICA, His,Avitag (Cat. No. MIA-H82E6) ELISA bioactivity

Immobilized Biotinylated Human MICA, His,Avitag (Cat. No. MIA-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human NKG2D, Fc Tag (Cat. No. NKD-H5265) with a linear range of 0.3-10 ng/mL (QC tested).

Biotinylated Human MICA, His,AvitagBiotinylated Human MICA, His,Avitag (Cat. No. MIA-H82E6) ELISA bioactivity

Immobilized Human NKG2D, Fc Tag (Cat. No. NKD-H5265) at 2 μg/mL (100 μL/well) can bind Biotinylated Human MICA, His,Avitag (Cat. No. MIA-H82E6) with a linear range of 0.3-39 ng/mL (Routinely tested).

  • Background
    MHC class I polypeptide-related sequence A (MICA) belongs to the MHC class I family and MIC subfamily. MICA contains one Ig-like C1-type (immunoglobulin-like) domain. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. MICA is ligand for the KLRK1/NKG2D receptor. MICA bind to KLRK1 leads to cell lysis.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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