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Your Position: Home > CD&BIOMARKER > IgG1 Fc > IG1-H52H9

Human IgG1 Fc (C103S, L117F, L118E, P214S) Protein, His Tag

  • Synonym
    IgG1
  • Source
    Human IgG1 Fc (C103S, L117F, L118E, P214S), His Tag (IG1-H52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 99 - Lys 330 (Accession # P01857-1(C103S, L117F, L118E, P214S)).
    Predicted N-terminus: Glu 99
  • Molecular Characterization
    Online(Glu 99 - Lys 330) P01857-1 (C103S, L117F, L118E, P214S)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IgG1 Fc (C103S, L117F, L118E, P214S), His Tag (Cat. No. IG1-H52H9) SDS-PAGE gel

Human IgG1 Fc (C103S, L117F, L118E, P214S), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human IgG1 Fc (C103S, L117F, L118E, P214S), His TagHuman IgG1 Fc (C103S, L117F, L118E, P214S), His Tag (Cat. No. IG1-H52H9) ELISA bioactivity

Immobilized Human IgG1 Fc (C103S, L117F, L118E, P214S), His Tag (Cat. No. IG1-H52H9) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.01-0.625 μg/mL (QC tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human IgG1 Fc (C103S, L117F, L118E, P214S), His Tag (Cat. No. IG1-H52H9) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 1.42 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Datasheet & Documentation

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DMF (Drug Master File)

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