Biotinylated Human NPR1 / NPRA Protein, His,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
NP1-H82E9-25ug
$360.00
NP1-H82E9-200ug
$1420.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Atrial natriuretic peptide receptor 1, NPR1, NPRA, GC-A, ANPRA, NPR-A

  • Source

    Biotinylated Human NPR1, His,Avitag (NP1-H82E9) is expressed from human 293 cells (HEK293). It contains AA Gly 33 - Glu 473 (Accession # P16066-1).

    Predicted N-terminus: Gly 33

    Request for sequence
  • Molecular Characterization

    NPR1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 52.5 kDa. The protein migrates as 63-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    NPR1 SDS-PAGE

    Biotinylated Human NPR1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    NPR1 SEC-MALS

    The purity of Biotinylated Human NPR1, His,Avitag (Cat. No. NP1-H82E9) is more than 85% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     NPR1 ELISA

    Immobilized Biotinylated Human NPR1, His,Avitag (Cat. No. NP1-H82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-NPR1 hIgG4 Antibody with a linear range of 0.06-2 ng/mL (QC tested).

    Protocol

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Background

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.

Recent Advances

 
Drug Development Progress
  • English Name:

    Natriuretic peptide receptor 1

  • Category:

  • Approved Drugs:

    4 Details

  • Drugs in Clinical Trials:

    7 Details

  • Highest Development Stage:

    Approved

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