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Your Position: Home > CKP > SIRP alpha > SIA-H5258

Human SIRP alpha / CD172a (31-149) Protein, Llama IgG2b Fc Tag, low endotoxin (MALS verified)

  • Synonym
    SHPS1,SIRPA,CD172A,BIT,MFR,MYD1,P84,PTPNS1
  • Source
    Human SIRP alpha (31-149), Llama IgG2b Fc Tag (SIA-H5258) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Ser 149 (Accession # P78324-1).
    Predicted N-terminus: Glu 31
  • Molecular Characterization
    Online(Glu 31 - Ser 149) P78324-1

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 40.8 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human SIRP alpha (31-149), Llama IgG2b Fc Tag (Cat. No. SIA-H5258) SDS-PAGE gel

Human SIRP alpha (31-149), Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human SIRP alpha (31-149), Llama IgG2b Fc Tag (Cat. No. ) MALS images

The purity of Human SIRP alpha (31-149), Llama IgG2b Fc Tag (Cat. No. SIA-H5258) is more than 90% and the molecular weight of this protein is around 73-98 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human SIRP alpha (31-149), Llama IgG2b Fc TagHuman SIRP alpha (31-149), Llama IgG2b Fc Tag (Cat. No. SIA-H5258) ELISA bioactivity

Immobilized Human SIRP alpha (31-149), Llama IgG2b Fc Tag (Cat. No. SIA-H5258) at 5 μg/mL (100 μL/well) can bind Human CD47, His Tag (Cat. No. CD7-H5227) with a linear range of 0.02-0.625 μg/mL (QC tested).

Human SIRP alpha (31-149), Llama IgG2b Fc TagHuman SIRP alpha (31-149), Llama IgG2b Fc Tag (Cat. No. SIA-H5258) ELISA bioactivity

Immobilized Human CD47, His Tag (Cat. No. CD7-H5227) at 5 μg/mL (100 μL/well) can bind Human SIRP alpha (31-149), Llama IgG2b Fc Tag (Cat. No. SIA-H5258) with a linear range of 0.2-10 ng/mL (Routinely tested).

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
  • Background
    Tyrosine-protein phosphatase non-receptor type substrate 1 (SHPS1) is also known as CD172 antigen-like family member A (CD172a), Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha (SIRPA or SIRP alpha) or p84, is a member of the SIRP family, and also belongs to the immunoglobulin superfamily. SIRP alpha is Ubiquitous and highly expressed in brain. SIRPA / CD172a is immunoglobulin-like cell surface receptor for CD47 and acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. SIRPA / SHPS-1 supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment and may play a key role in intracellular signaling during synaptogenesis and in synaptic function By similarity. SIRPA / MyD1 involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin and mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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