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Your Position: Home > Cytokines > IL-3 > IL3-C52H9

Cynomolgus IL-3 Protein, His Tag

  • Synonym
    IL3,MCGF,MGC79398,MGC79399,MULTI-CSF,Interleukin-3
  • Source
    Cynomolgus IL-3, His Tag(IL3-C52H9) is expressed from human 293 cells (HEK293). It contains AA Ala 20 -Gln 143 (Accession # G7P877-1 ).
    Predicted N-terminus: Ala 20
  • Molecular Characterization
    IL-3 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 15.9 kDa. The protein migrates as 18-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-3 SDS-PAGE

Cynomolgus IL-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-BLI
 IL-3 BLI

Loaded Human IL-3 R alpha, Fc Tag (Cat. No. ILA-H5252) on Protein A Biosensor, can bind Cynomolgus IL-3, His Tag (Cat. No. IL3-C52H9) with an affinity constant of 1.30 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

  • Background
    Interleukin 3 is also known as IL-3, is a protein that in humans is encoded by the IL3 gene. Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) that can improve the bodys natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. [3] Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 . Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats, and the PI3K/Akt pathway. IL-3 may play an important role in the development of airway inflammation associated with asthma. The human IL-3 gene encodes a protein 152 amino acids long, and the naturally occurring IL-3 is glycosylated. The human IL-3 gene is located on chromosome 5, only 9 kilobases from the GM-CSF gene, and its function is quite similar to GM-CSF.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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