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Your Position: Home > CAR-T > FMC63 > CD9-M52Hb

FMC63 scFv, His Tag (SPR verified)

  • Synonym
    Anti-FMC63 Ab & Anti-FMC63 scFv Antibody
  • Source
    FMC63 scFv, His Tag (CD9-M52Hb) is expressed from human 293 cells (HEK293). It mimics the scFv presented on CAR-T cells which target CD19.
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 29.0 kDa. The protein migrates as 29 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20 to -70°C for 12 months in lyophilized state from date of receipt;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FMC63 SDS-PAGE

FMC63 scFv, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 FMC63 ELISA

Immobilized Human CD19 (20-291), Fc Tag (Cat. No. CD9-H5251) at 5 μg/mL (100 μL/well) can bind FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) with a linear range of 0.6-39 ng/mL (QC tested).

Bioactivity-SPR
 FMC63 SPR

Human CD19 (20-291), Fc Tag (Cat. No. CD9-H5251) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind FMC63 scFv, His Tag (Cat. No. CD9-M52Hb) with an affinity constant of 0.668 nM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $190.00

Price(USD) : $1380.00

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Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

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