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Your Position: Home > Protein > Spike protein > SPN-B52H4

Bat coronavirus RaTG13 Spike Trimer Protein (R681A, KV982-983PP), His Tag

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  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    Bat coronavirus RaTG13 Spike Trimer, His Tag (SPN-B52H4) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Pro 9 - Pro 1209 (Accession # A0A6B9WHD3-1 (R681A, KV982-983PP)).
    Predicted N-terminus: Pro 9
  • Molecular Characterization
    Spike protein Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 138.2 kDa. The protein migrates as 170-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike protein SDS-PAGE

Bat coronavirus RaTG13 Spike Trimer, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
 Spike protein ELISA

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind Bat coronavirus RaTG13 Spike Trimer (R681A, KV982-983PP), His Tag (Cat. No. SPN-B52H4) with a linear range of 0.016-2 μg/mL (Routinely tested).

  • Background
    The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

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DMF (Drug Master File)

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