Save Product
Product Details
Synonyms
SHP-2
Source
Human SHP-2, His Tag (SH2-H5248) is expressed from E. coli cells. It contains AA Thr 2 - Arg 593 (Accession # Q06124-2).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 69.3 kDa. The protein migrates as 65-66 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human SHP-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity
Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site. The specific activity is >3 umol/min/mg, measured under the described conditions (QC tested).
Protocol
Serial dilutions of SHP394 (an orally efficacious inhibitor of protein tyrosine phosphatase SHP2) were added into Human SHP-2 Protein, His Tag(SH2-H5248) enzymatic reactions. The half maximal inhibitory concentration (IC50) is 19.36 nM (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Src homology phosphotyrosyl phosphatase 2
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
21 Details
- Highest Development Stage：
Phase 3 Clinical
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
