Product Details
Synonyms
NKG2A & CD94
Source
Biotinylated Cynomolgus NKG2A&CD94, Fc,Avitag (NC4-C82F7) is expressed from human 293 cells (HEK293). It contains AA Ala 113 - Leu 233 (NKG2A) & Asp 57 - Ile 179 (CD94) (Accession # Q68VD2 (NKG2A) & Q68VD4 (CD94)).
Predicted N-terminus: 0
Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 56.8 kDa. The protein migrates as 65-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Cynomolgus NKG2A&CD94, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Biotinylated Cynomolgus NKG2A&CD94, Fc,Avitag (Cat. No. NC4-C82F7) is more than 90% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Anti-NKG2A Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus NKG2A&CD94, Fc,Avitag (Cat. No. NC4-C82F7) with a linear range of 0.1-16 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Cynomolgus NKG2A&CD94, Fc,Avitag (Cat. No. NC4-C82F7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind various dilution ratio of HLA-E*01:03 HLA-A leader3-11 Tetramer-VMAPRTLVL-PE (Routinely tested).
Protocol
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Background
Recent Advances
- English Name:
NK cell lectin-like receptor subfamily C member 1
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
6 Details
- Highest Development Stage:
Phase 3 Clinical
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