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Your Position: Home > Cytokines > Complement C2 > CO2-C52H8

Cynomolgus Complement C2 Protein, His Tag (active enzyme)

  • Synonym
    C2,Complement C2,Complement Component C2
  • Source
    Cynomolgus Complement C2, His Tag(CO2-C52H8) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Leu 752 (Accession # G8F3W0-1 ).
    Predicted N-terminus: Ala 21
  • Molecular Characterization
    Complement C2 Structure

    This protein carries a polyhistidine tag at the C-terminus

    The protein has a calculated MW of 83.0 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris,150 mM NaCl,Ph 7.5 with Trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Complement C2 SDS-PAGE

Cynomolgus Complement C2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB).The specific activity is >100 pmol/min/µg (QC tested).

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