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Your Position: Home > Antibody > FMC63 > FM3-PY54A2

PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (Site-specific conjugation) (0.03% Proclin)DMF

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  • Source
    PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 is produced via site-specific conjugation of PE to Monoclonal Anti-FMC63 Antibody, Mouse IgG1 under optimal conditions with a proprietary technology.
    We carry another premium grade PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) (FM3-PY54G0), produced with the same production process but under more rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. It is designed for cell isolation and cell culture applications in the early preclinical stage.
  • Application
    Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression). Please note that this product is NOT compatible to streptavidin detection system.
  • Clone
    Y45
  • Host Species
    Mouse
  • Isotype
    Mouse IgG1/kappa
  • Specificity
    Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.
  • Reactivity

    Mouse

  • Immunogen

    Recombinant FMC63 scFv derived from HEK293 cells.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Isotype Control

    The Isotype control is sold separately and you can search for Cat. No. DNP-PM1 for product information.

  • Recommended Dilution
    1:50
  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, 0.03% Proclin, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 12 months after reconstitution;
    3. 2-8°C for 12 months after reconstitution.
Bioactivity-FACS
 FMC63 FACS

5e5 of anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-PY54A2) and isotype control antibody respectively. PE signal was used to evaluate the binding activity (QC tested).

 FMC63 FACS

Binding activity of the PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 before and after adding 0.03% Proclin was evaluated in the above FACS analysis. The result shows that PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-PY54A2) and PE-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Cat. No. FM3-HPY53) have the same binding activity against anti-CD19 CAR-293 cells.

  • Background
    FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.
  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $377.00

Price(USD) : $2287.00

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