Pangolin coronavirus isolate PCoV_GX-P5L Spike Trimer Protein (R679A, KV980-981PP), His Tag (MALS verified)

Synonyms

Spike, S protein, Spike glycoprotein, S glycoprotein

Source

Pangolin coronavirus isolate PCoV_GX-P5L Spike Trimer, His Tag (SPN-P52H4) is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. It contains AA Pro 9 - Pro 1207 (Accession # A0A6G6A1M4 (R679A, KV980-981PP)).

Predicted N-terminus: Pro 9

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 138.2 kDa. The protein migrates as 170-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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