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Product Details
Synonyms
IL-17 RC, IL-17RC, IL17Rhom, IL-17 receptor C, IL-17RL, ZcytoR14
Source
Biotinylated Human IL-17 RC, Fc,Avitag (ILC-H82F9) is expressed from human 293 cells (HEK293). It contains AA Leu 21 - His 465 (Accession # Q8NAC3-2).
Predicted N-terminus: Leu 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 77.6 kDa. The protein migrates as 80-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human IL-17 RC, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human IL-17 RC, Fc,Avitag (Cat. No. ILC-H82F9) is more than 85% and the molecular weight of this protein is around 215-270 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL17A, Tag Free (Cat. No. ILA-H5219) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RC, Fc,Avitag (Cat. No. ILC-H82F9) with a linear range of 0.01-0.156 μg/mL (QC tested).
Protocol
Immobilized Human IL-17F, His Tag (Cat. No. ILF-H5244) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RC, Fc,Avitag (Cat. No. ILC-H82F9) with a linear range of 0.01-0.078 μg/mL (Routinely tested).
Protocol
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