Rat ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free, has a calculated MW of 113 kDa (ITGAV) & 77 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl,pH 7.5 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free (Cat. No. IT8-R52E1) is more than 90% and the molecular weight of this protein is around 200-230kDa verified by SEC-MALS.
Immobilized Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free (Cat. No. IT8-R52E1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.2-13 ng/mL (QC tested).
Immobilized Rat Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer, His Tag&Tag Free (Cat. No. IT8-R52E1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Vitronectin, Tag Free, primary amine labeling (Cat. No. VIN-H8113) with a linear range of 0.1-3 ng/mL (Routinely tested).
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