This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 36.2 kDa. The protein migrates as 14 kDa and 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, 5 mM CHAPS, 1 mM PMSF, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human APOE4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human APOE4, His Tag (Cat. No. APE-H52H9) is more than 90% and the molecular weight of this protein is around 178-193 kDa verified by SEC-MALS.
Loaded Human TREM2, Mouse IgG2a Fc Tag (Cat. No. TR2-H5256) on Protein A Biosensor, can bind Human APOE4, His Tag (Cat. No. APE-H52H9) with an affinity constant of 37.6 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Human TREM2, Fc Tag (Cat. No. TR2-H5254) on Protein A Biosensor, can bind Human APOE4, His Tag (Cat. No. APE-H52H9) with an affinity constant of 85.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human TREM2, His,Avitag (Cat. No. TR2-H82E7) on SA Biosensor, can bind Human APOE4, His Tag (Cat. No. APE-H52H9) with an affinity constant of 89.0 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human LDL R, Fc Tag (Cat. No. LDR-H5254) on Protein A Biosensor, can bind Human APOE4, His Tag (Cat. No. APE-H52H9) with an affinity constant of 42.8 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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